The Habituation/Cross-Habituation Test Revisited: Guidance from Sniffing and Video Tracking

Figure 14

New combined proximity-sniffing criterion improves HaXha exploration scoring in mice. To demonstrate the usefulness of using sniff rate itself as an exploration criterion for WT (left, ) and FUS KO (right, ) mice, bins were scored using sniff rate >8.2 Hz (the 97.5th percentile of sniff_OFFdi4Xsniff, i.e., unusually high sniff rates when mice are not exploring) and distance <4 cm. Sniff rate (Hz) when exploring (sniff_ON_di4Xsniff) and not exploring (sniff_OFF_di4Xsniff) and exploration time (s; explore_ON_di4Xsniff) are shown. The difference (%) in time bins meeting this new and the standard criterion was also assessed (% overlap; crit_diffpct_div4sniff). (paired 1-sided -test versus S1).