Research Article

The Habituation/Cross-Habituation Test Revisited: Guidance from Sniffing and Video Tracking

Figure 8

Distance and velocity combined predict sniff rates. Population vectors that include all time bins from all WT mice were used to regress both nose to odor source distance and head angle to odor source (dist-angle) or distance and velocity (dist-veloc) parametrically onto sniff rates using 1st- or 2nd-order equations (shown in Figure 9), for either all trials of WT or FUS KO mice or their S1 trials. The 2nd-order distance-velocity regression explained 43–60% of sniff rates (adjusted ; S1: mice 1201 time bins = 8407 bins; all: mice 1201 bins 4 trials = 33,628 time bins). A 3-way stepwise 1st-order multiple regression (dist-angle-veloc 1st, right) also robustly explained sniff rates.