Wnt5a Increases the Glycolytic Rate and the Activity of the Pentose Phosphate Pathway in Cortical Neurons

Figure 4

Wnt pathway activation stimulates the glucose metabolism in hippocampal slices. (a) Schematic representation of 350 m hippocampal slice. (b) In slices, Wnt5a treatment induced an increase in the intracellular accumulation of 2-DG in a time-dependent manner (i); this effect was blocked by the coincubation with FzdCRD-2 (ii). (c) Slices incubated with Wnt5a showed an increase in the glycolytic rate; this effect was blocked by 2-DG and FzCRD-2. (d) In slices we observed an increase in the PPP activity. Data represent the mean ± SEM of , each performed in triplicate. ; , Bonferroni test.

(a)

(b)

(c)

(d)