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Figure 3: AAV-KLF7 significantly increased the expression of KLF7 and target proteins in vitro. (a) Representative Western blot analysis of KLF7, NGF, and TrkA expression in the cultured spinal cord neurons of the three groups is shown at 4 days in vitro (6 wells/each group). The graphs represent the relative density of KLF7 (b), NGF (c), and TrkA (d) protein. (e) Representative RT-PCR analysis of KLF7, NGF, and TrkA expression in the cultured spinal cord neurons of the three groups is shown at 4 days in vitro (). The graphs represent the relative density of KLF7 (f), NGF (g), and TrkA (h) mRNA. Error bars denote SD. , , and . NS: not significant. One-way ANOVA, Tukey’s post hoc test.