Pilocarpine-Induced Status Epilepticus Is Associated with Changes in the Actin-Modulating Protein Synaptopodin and Alterations in Long-Term Potentiation in the Mouse Hippocampus

Figure 3

Pilocarpine-induced status epilepticus affects long-term potentiation (LTP) of Schaffer collateral-CA1 synapses. (a–c) LTP of Schaffer collateral-CA1 synapses was probed in acute dorsal hippocampal slices. A single 100 Hz tetanus (1 s) is applied (stim), to induce LTP (recordings of field excitatory postsynaptic potentials; fEPSP) in slices prepared from either pilocarpine- or vehicle-injected animals (stratum radiatum, rad; stratum lacunosum-moleculare, la.-mol.; stratum moleculare, mL; dentate gyrus, DG; nuclear stain, TO-PRO; scale bar: 200 μm). While no significant difference in fiber volleys and input/output properties is detected (b), a deficit in the ability to induce LTP at Schaffer collateral-CA1 synapses is observed in the pilocarpine group (c). Values normalized to baseline fEPSP slope. = , = ; ; slices (1 slice per animal) of each group. -test (unpaired, two-tailed).