Pathological Role of Peptidyl-Prolyl Isomerase Pin1 in the Disruption of Synaptic Plasticity in Alzheimer’s Disease

Figure 2

Pin1 proteins are partly colocalized with Shank3 and NR1 proteins at dendritic spines of neurons. Cortical neurons at 21 DIV were double immunolabeled for Pin1 (red) and Shank3 (green) or NR1 (green). When the images were merged, the yellow in the overlay showed colocalization of Pin1-immunoreactive puncta with Shank3 and NR1 proteins. Right panels were high-magnification images of selected overlays.