Could Perinatal Asphyxia Induce a Synaptopathy? New Highlights from an Experimental Model

Figure 2

Microphotographs of synaptic terminals of the hippocampus CA1 area from animals subjected to PA. (a–e) Hippocampal synapses stained with E-PTA showing increased thickness in PSDs. (f) More consistent changes were observed in PSDs after 180 days of PA. Three-dimensional reconstruction of the asphyctic postsynaptic domain (g) showed clear signs of degeneration. (h) Dendritic shaft injected with Golgi silver staining showed filopodium after 30 days of PA (arrows). (i-j) DS stained with phalloidin eosin and photoconverted. A higher number of DS are observed at 30 days compared with 180 days after PA. PSDs = postsynaptic densities; E-PTA = ethanolic phosphotungstic acid; DS = dendritic spines. Scale bar 0.5 μm.

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