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Neural Plasticity
Volume 2017, Article ID 4153076, 16 pages
https://doi.org/10.1155/2017/4153076
Research Article

Mechanisms and Consequences of Dopamine Depletion-Induced Attenuation of the Spinophilin/Neurofilament Medium Interaction

1Department of Biology, Indiana University-Purdue University Indianapolis, 723 W. Michigan St., Indianapolis, IN 46202, USA
2Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, 723 W. Michigan St., Indianapolis, IN 46202, USA
3Neuroscience Undergraduate Program, Indiana University-Purdue University Indianapolis, 723 W. Michigan St., Indianapolis, IN 46202, USA
4Department of Psychology, Indiana University-Purdue University Indianapolis, 723 W. Michigan St., Indianapolis, IN 46202, USA
5Molecular Physiology and Biophysics, Vanderbilt University School of Medicine University, 724 Robinson Research Building, 23rd Ave South at Pierce, Nashville, TN 37232, USA
6Department of Biochemistry and the Mass Spectrometry Research Center, Vanderbilt University School of Medicine University, 465 21st Ave S. Room 9160, MRB III, Nashville, TN 37232, USA
7Stark Neurosciences Research Institute, Indiana University School of Medicine, Indiana, IN, USA

Correspondence should be addressed to Anthony J. Baucum II; ude.iupui@mucuabja

Received 9 January 2017; Accepted 16 February 2017; Published 28 May 2017

Academic Editor: Tomasz Wójtowicz

Copyright © 2017 Andrew C. Hiday et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table (S1) Spectral counts of proteins containing at least 48 spectral counts across 6 biological replicates. The "Spectral Counts and Ratios" tab shows the spectral counts from each fraction and each replicate. The "Ratios Only" tab shows the total number of spectral counts from the S2 and S3 fraction for each protein across the 6 biological replicates. Both tabs also show a ratio that was calculated by dividing the number of spectral counts isolated from the lesioned hemisphere by the number of spectral counts isolated from the intact hemisphere. This calculation was performed independently for each subcellular fraction. Supplemental Figure S1. Sample MS/MS spectra matching NF-M phosphopeptides that were detected in the NF-M immunoprecipitates isolated from HEK293 cells. Supplemental Figure S2. NF-M sequence in the KSP-repeat domain with tryptic peptides that were identified in the NF-M immunoprecipitates isolated from HEK293 cells.

  1. Supplementary material