Figure 8: RGFP966 inhibited LPS-induced microglia activation. (a) Representative microphotographs of primary microglia cultures 24 hours after LPS and RGFP966 treatment. The cultures were stained with CD16/32, a marker of activated microglia. Scale bar = 10 μm. (b) Expression and phosphorylation of STAT3 and STAT5 in microglia pretreated with RGFP966. (c) Quantitative analysis of fluorescence intensity of CD16/32. All the photographs used for quantitative analysis were taken under the same exposure time and data were expressed as fluorescence intensity per cell of randomly selected regions. . repeats. Supernatant concentration of IL-6 (d) and TNF-α (e) detected at various stimulation times. , . repeats.