Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase

Figure 4

JNK preferentially interacts with Syntaxin-1, Syntaxin-2, and Snap25. (a–c) Western blotting after immunoprecipitation of Syntaxin-1 (a), Syntaxin-2 (b), and Snap25 (c) on purified synaptosomes lysates extracted from adult mice cortex. JNK is detectable in Syntaxin-1 and Syntaxin-2 precipitate, while immunoreactive signal is weak in Snap25 precipitate. (d) Both Syntaxin-1 and Syntaxin-2, as well as Snap25, were detectable in JNK precipitate. 500 g of total lysates has been subjected to immunoprecipitation, while 20 g was loaded as control. .

(a)

(b)

(c)

(d)