Tail Nerve Electrical Stimulation and Electro-Acupuncture Can Protect Spinal Motor Neurons and Alleviate Muscle Atrophy after Spinal Cord Transection in Rats

Figure 4

Spinal neurons expressed more excitatory neurotransmitter synthetase—ChAT in L5 spinal cord after TANES or EA treatment. (a) The expression of ChAT in L5 spinal cord is detected with Western blot among the four groups. (b) Quantitative analysis of data in (a). Data are presented as mean ± SD. , as compared with the SCI group; ^#, as compared with the Sham group. (c, d, e, f) ChAT (green) and NeuN (red) double-label immunofluorescence staining shows the expression of ChAT in L5 motor neurons from the SCI, TANES, EA, and Sham groups. Scale bars = 100 μm in (c, d, e, f).

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