Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses

Figure 5

Startle response of fish larvae with neomycin treatment of different level of concentration and subsequent recovery period after drug removal. Mean moving distance after sound stimulation was used as the quantification parameter. To eliminate the variation of different days, the values are scaled by that of the control group in each day. The value in each column from left to right is , , , , , , , 1.10 ± 0.19, or 0.72 ± 0.34 in the format of mean ± standard deviation. The unpaired t-test on adjacent columns in each day is shown to be mostly significant, except for between control and 0.16 μM at 24 h or at 72 h. For the same drug concentration (e.g., 0.16 μM at 0 h, 24 h, and 72 h), unpaired t-test shows significant () difference between days for both 0.16 and 1.6 μM cases.