Effects of Propofol Treatment in Neural Progenitors Derived from Human-Induced Pluripotent Stem Cells

Figure 2

High-concentration propofol treatment exerts toxicity to NPCs. NPCs derived from three hiPSC lines were exposed to propofol at different concentrations (0, 20, 50, 100, and 300 μM). MTT assays showed high-concentration propofol-reduced NPCs viability after the 6 h (a) or 24 h (c) treatment, or 6 h treatment followed by 20 h washout (b). Data from LDH assays further showed that propofol at a high concentration reduced the number of NPCs (d). Data at each concentration were analyzed by unpaired t-tests. For MTT assays, data were expressed as a percentage of viable cells of the treatment compared to the untreated (0 μM propofol) group (mean ± SD). For LDH assays, data were expressed as a percentage of positive control (mean ± SD), compared to 0 μM propofol exposure group. For both assays, 5 wells per treatment condition were examined. Each experiment was repeated for at least three times. ; ; .

(a)

(b)

(c)

(d)