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Neural Plasticity
Volume 2017 (2017), Article ID 9857918, 12 pages
Research Article

Electrophysiological, Morphological, and Ultrastructural Features of the Injured Spinal Cord Tissue after Transplantation of Human Umbilical Cord Blood Mononuclear Cells Genetically Modified with the VEGF and GDNF Genes

1Kazan Federal University, Kazan, Russia
2Kazan State Medical University, Kazan, Russia
3Republic Clinical Hospital, Kazan, Russia
4Kazan State Academy of Veterinary Medicine, Kazan, Russia

Correspondence should be addressed to Y. O. Mukhamedshina; ur.liam@n-z-k.anay

Received 19 September 2016; Accepted 24 January 2017; Published 21 March 2017

Academic Editor: J. Michael Wyss

Copyright © 2017 Y. O. Mukhamedshina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this study, we examined the efficacy of human umbilical cord blood mononuclear cells (hUCB-MCs), genetically modified with the VEGF and GDNF genes using adenoviral vectors, on posttraumatic regeneration after transplantation into the site of spinal cord injury (SCI) in rats. Thirty days after SCI, followed by transplantation of nontransduced hUCB-MCs, we observed an improvement in (latency period, LP) and waves, compared to the group without therapy after SCI. For genetically modified hUCB-MCs, there was improvement in of wave and LP of both the and waves. The ratio between of the and waves () demonstrated that transplantation into the area of SCI of genetically modified hUCB-MCs was more effective than nontransduced hUCB-MCs. Spared tissue and myelinated fibers were increased at day 30 after SCI and transplantation of hUCB-MCs in the lateral and ventral funiculi 2.5 mm from the lesion epicenter. Transplantation of hUCB-MCs genetically modified with the VEGF and GNDF genes significantly increased the number of spared myelinated fibers (22-fold, ) in the main corticospinal tract compared to the nontransduced ones. HNA+ cells with the morphology of phagocytes and microglia-like cells were found as compact clusters or cell bridges within the traumatic cavities that were lined by GFAP+ host astrocytes. Our results show that hUCB-MCs transplanted into the site of SCI improved regeneration and that hUCB-MCs genetically modified with the VEGF and GNDF genes were more effective than nontransduced hUCB-MCs.