Activation of Phosphotyrosine-Mediated Signaling Pathways in the Cortex and Spinal Cord of SOD1^G93A, a Mouse Model of Familial Amyotrophic Lateral Sclerosis

Figure 1

Activation of phosphotyrosine signal in the cortex and spinal cord of SOD1^G93A mice. Synaptosomes (1 mg/ml) were prepared from the motor cortex (a) and spinal cord (b) of control, SOD1^WT, and SOD1^G93A animals, and phosphotyrosine content was evaluated in solubilized synaptosomes by Western blot (WB) analysis using an anti-pY antibody. The nitrocellulose was also probed with an anti-β-actin antibody to evaluate the amount of loaded proteins (lower panels). Immunoreactive bands were detected by ECL. Data are representative of four separate experiments.

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