Neural Plasticity / 2018 / Article / Fig 1

Research Article

Activation of Phosphotyrosine-Mediated Signaling Pathways in the Cortex and Spinal Cord of SOD1G93A, a Mouse Model of Familial Amyotrophic Lateral Sclerosis

Figure 1

Activation of phosphotyrosine signal in the cortex and spinal cord of SOD1G93A mice. Synaptosomes (1 mg/ml) were prepared from the motor cortex (a) and spinal cord (b) of control, SOD1WT, and SOD1G93A animals, and phosphotyrosine content was evaluated in solubilized synaptosomes by Western blot (WB) analysis using an anti-pY antibody. The nitrocellulose was also probed with an anti-β-actin antibody to evaluate the amount of loaded proteins (lower panels). Immunoreactive bands were detected by ECL. Data are representative of four separate experiments.
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