Activation of Phosphotyrosine-Mediated Signaling Pathways in the Cortex and Spinal Cord of SOD1G93A, a Mouse Model of Familial Amyotrophic Lateral Sclerosis
Tyrosine phosphorylation of STEP substrates. The tyrosine phosphorylation levels of GluN2B (a), ERK1/2 (b), and Pyk2 (c) were evaluated by Western blot analysis using specific antibodies that recognized the phosphorylated and nonphosphorylated forms of each enzyme in the cortex and spinal cord of control and SOD1G93A animals. Anti-β-actin antibody was used to evaluate the amount of loaded proteins. The immunoreactive bands were detected by ECL. The immunoblots are representative of three independent experiments. The bar graphs represent quantification by densitometric analysis of band intensity relative to the appropriate nonphosphorylated proteins, expressed as percentage of the relative controls. White and black columns: control and SOD1G93A mice. The bar graph represents the means ± SEM of 3 independent experiments. Significantly different from control (, Student’s -test).
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