Environmental Enrichment Induces Changes in Long-Term Memory for Social Transmission of Food Preference in Aged Mice through a Mechanism Associated with Epigenetic Processes

Figure 3

(a) c-fos expression in subregions of OFC for SC (open columns), EE (filled columns), PL-SC (dotted columns), and PL-EE (hatched columns) mice subjected to choice phase at day 1 and day 30. MO: two-way ANOVA, factor condition (), factor time (), condition × time (), post hoc analysis Holm-Sidak method. SC versus PL-SC and PL-SC versus PL-EE () for all retention intervals. Statistical differences were found between EE versus PL-EE at day 1 () and day 30 (); EE versus SC significant at day 30 (). LO: two-way ANOVA, factor condition (), factor time (), condition × time (), post hoc analysis Holm-Sidak method. PL-SC versus PL-EE () for all retention intervals. Statistical differences were found between SC versus PL-SC at day 1 (); EE versus PL-EE at day 1 () and day 30 (); EE versus SC significant at day 30 (). Statistical differences within group were found between day 1 and day 30 for SC group () and for EE group (). VO: two-way ANOVA, factor condition (), factor time (), condition × time (), post hoc analysis Holm-Sidak method. SC versus PL-SC and PL-SC versus PL-EE () for all retention intervals. Statistical differences were found between EE versus PL-EE at day 1 () and day 30 (); EE versus SC significant at day 30 (). Statistical differences within EE group were found between day 1 and day 30 (). DLO: two-way ANOVA, factor condition (), factor time (), condition × time (), post hoc analysis Holm-Sidak method. PL-SC versus PL-EE () for all retention intervals. Statistical differences were found between SC versus PL-SC at day 1 (); EE versus PL-EE at day 1 () and day 30 (); EE versus SC significant at day 30 (). Statistical differences within EE group were found between day 1 and day 30 (). ; ; ; error bars = SEM. (b) Representative panel of c-fos protein expression in DLO for SC, EE, PL-SC, and PL-EE mice, following recall at day 1 and day 30; scale bar: 50 μm.

(a)

(b)