Antidepressant Effects of Probucol on Early-Symptomatic YAC128 Transgenic Mice for Huntington’s Disease

Figure 1

Experimental protocol. Thirty-day-old WT and YAC128 female and male mice received probucol (30 mg/kg/day) or vehicle (1% CMC) in drinking water for 5 months. Two distinct cohorts of animals were used. Animals from Cohort 1 were submitted to the tail suspension test (TST) and open field test (OFT) at 2, 4, and 6 months of age and to the forced swimming test (FST) and the rotarod test at 4 and 6 months. 24 h after the last behavioral test, mice were transcardially perfused; their brains were removed and processed for immunohistochemistry analyses of cell proliferation and neuronal differentiation. Animals from Cohort 2 were subjected to the same treatment regime and euthanized 24 hours after the last behavioral test to determine cholesterol plasma levels.