Ropinirole and Pramipexole Promote Structural Plasticity in Human iPSC-Derived Dopaminergic Neurons via BDNF and mTOR Signaling

Table 1

Statistical analysis of the effects produced by D3R antagonists, by MEK-ERK and PI3K-mTOR inhibitors, and by BDNF-TrkB signaling inhibitors on ropinirole-induced structural plasticity in F3 DA neurons.

Experiments	Two-way ANOVA	Max dendrite length	Number primary dendrites	Soma area
(DA antag.)^1 X (Rop/Veh)	Interaction	F(4,290) = 5.4	F(4,490) = 4.9	F(4,390) = 4.3
	Antagonist factor	F(4,290) = 6.8	F(4,490) = 2.8	F(4,390) = 3.8
	Ropinirole factor	F(1,290) = 7.6	F(1,490) = 7.2	F(1,390) = 7.7
(mTOR inh.)^2 X (Rop/Veh)	Interaction	F(3,232) = 3.5	F(3,392) = 4.9	F(3,312) = 2.9
	Inhibitor factor	F(3,232) = 4.5	F(3,392) = 3.7	F(3,312) = 4
	Ropinirole factor	F(1,232) = 7.7	F(1,392) = 4	F(1,312) = 6.1
(TrkB inh.)^3 X (Rop/Veh)	Interaction	F(4,290) = 3.5	F(4,490) = 5.4	F(4,390) = 2.9
	Inhibitor factor	F(4,290) = 6.3	F(4,490) = 2.8	F(4,390) = 4.1
	Ropinirole factor	F(1,290) = 5.7	F(1,490) = 4.4	F(1,390) = 10

¹DA antagonists: sulpiride, SB277011-A, S33084, and SCH23390. ²mTOR inhibitors: PD98059, LY294002, and rapamycin. ³TrkB inhibitors: α-BDNF, TrkB-Fc Chimera, K252a, and PP2. The original values represented as mean ± SEM can be found in Figure 4. Two way ANOVA: ; ; and .