Newly Generated Cells in the Dentate Gyrus Differentially Respond to Brief Spatial Exploration and Forced Swim in Adult Female Balb/C Mice

Figure 5

Activation of newborn cells in the dentate gyrus of adult mice. (a) Representative micrographs of IdU/Arc- and CldU/Arc-labelled cells are shown. IdU or CldU (green), Arc (red), and the merged micrographs are shown. The scale bar is equal to 10 μm. (b–c) Quantification of IdU- and CldU-activated (Arc) cells in mice exposed to brief spatial exploration is shown. The number of newborn activated (Arc) cells was calculated by multiplying the ratio of newborn activated IdU or CldU cells with the absolute number of IdU- or CldU-labelled cells, respectively. (b) The number of IdU/Arc-labelled cells increased in mice exposed to environment A once or twice in comparison to that in mice subsequently exposed to environments A and B. Asterisks indicate . However, an increase in the number of CldU/Arc-labelled cells was found in mice exposed to environment A twice or in mice subsequently exposed to environment (a and b) in comparison to that in mice exposed to environment A or the control group (c). Asterisks indicate and , respectively. Data are expressed as the , and comparisons were performed with a one-way ANOVA followed by Student-Newman-Keuls post hoc test. per group. (d, e) The number of IdU/Arc-labelled cells was significantly lower compared to mice exposed to forced swim (FS) than that found in mice not exposed to forced swim (No-FS). However, CldU/Arc-labelled cells were significantly higher in mice exposed to forced swim (FS). The asterisk in (d) indicates . The asterisk in (e) indicates . per group. Data are expressed as the , and comparisons were performed with unpaired Student’s -test.

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