Contextual Fear Memory Formation and Destabilization Induce Hippocampal RyR2 Calcium Channel Upregulation

Figure 2

Contextual fear training promotes RyR2 upregulation in situ. (a) Representative confocal images of RyR2 immunofluorescence (green) and nuclear stain (blue) obtained from the CA1, CA3, and DG hippocampal regions. Samples were obtained 5 h after training animals (T5) in the contextual fear protocol or from controls similarly exposed to the context in the absence of the electric shock. Scale bar: 20 μm. (b) Normalized RyR2 immunofluorescence values. To calculate these values, RyR2 immunofluorescence in the entire image was divided in each case by the corresponding nuclear stain, and the control ratios were set as 1. Values represent mean ± SE; for the T5 and the control groups. Statistical analysis was performed with paired one-tailed Student’s -test. ; ns: not significant.

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