Contextual Fear Memory Formation and Destabilization Induce Hippocampal RyR2 Calcium Channel Upregulation

Figure 3

Context reexposure for 3, 15, or 30 min induces memory extinction and enhances RyR2 expression. (a, c, and e) Memory retention was evaluated 24 h after training (T24) or 5 h after reexposure to the context for 3 min (R3), 15 min (R15), or 30 min (R30). Control rats did not receive electrical stimulation during the training session and were evaluated 5 h after reexposure to the context for 3 min, 15 min, or 30 min. (b, d, and f) RyR2 expression was analyzed 5 h after performing the respective reexposure sessions (see scheme at left). Representative blots and quantification (bar graphs) showing hippocampal RyR2 protein content in each group of rats. Values represent mean ± SE. The number of independent determinations is indicated in each graph. Statistical analysis in (a, c, and e) was done with one-way ANOVA followed by Tukey’s multiple comparison post hoc test; statistical analysis in (b, d, and f) was performed with Student’s -test. , , .