Guiding Lights in Genome Editing for Inherited Retinal Disorders: Implications for Gene and Cell Therapy

Figure 4

Schematic representation of the structure of a zinc finger nuclease (ZFN) and transcription activator-like effector nucleases (TALENs). (a) Cartoon of a ZFN dimer bound to DNA. ZFNs consist of two functional domains. A DNA-binding domain composed of three zinc finger modules, each one recognizing a unique triplet (3 bp) in the DNA. The DNA-cleaving domain composed of the FokI nuclease is attached to the zinc finger modules and induces the DSB in the DNA. (b) Cartoon of a TALEN dimer bound to DNA. TALENS bind DNA using the TAL effector recognizing individual nucleotides forming the DNA-binding domain. In addition, a DNA-cleaving domain comprised of the FokI nuclease is also present and will induce the DSB at the precise location in the DNA.

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