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| Animal model (age) | Experimental protocol | Structure analyzed | Method | Timing | NMDAR changes | Ref. |
|
| Adult Sprague-Dawley rats | LTP in vivo in the dentate gyrus | Dentate gyrus | WB of whole homogenates | 0, 0.20, 1, 4, and 48 hs and 2 weeks after LTP induction | Increase of GluNB and GluN2A at 20 and 48 hs after LTP induction. | [28] |
| Sprague-Dawley rats (6–8 weeks) | LTP induction in CA1 minislices | Hippocampal CA1 region | (i) WB of synaptosomal membrane and light membrane fractions from minislices | 30 min after LTP induction (subcellular fractionation assay) | Enhanced surface expression of GluN1 and GluN2A at 30 min after stimulation, with a significant decrease in the intracellular pools. There is no change in total NMDAR level (both methods). | [33] |
| (ii) WB of BS3-treated homogenates (cross-linking assay) from minislices | 0, 30, 60, 90, 120, 150, and 180 min (cross-linking assay) | The increase of GluN1 and GluN2A starts at 15 min and persists for at least 3 h after LTP induction (cross-linking assay). |
| Rats (6-7 days) | LTP induction in organotypic cultures of hippocampal slices | Hippocampal CA1 region | Whole-cell recordings | | GluN2A-NMDAR increase. | [32] |
| Adult Sprague-Dawley rats | LTP in vivo in the dentate gyrus | Dentate gyrus | WB of whole homogenates and synaptoneurosomes | 0.20, 4, 8, and 48 h and 2 weeks after LTP induction (whole homogenates assay) | Increase of GluN1 at 8 and 48 h after LTP induction (whole homogenate assay). | [29] |
| 0.20 and 48 h (synaptoneurosomes assay) | Increase of GluN2B at 0.20 and 48 h following LTP induction. Increase of GluN1 at 48 h, but not at 20 min, post LTP induction (synaptoneurosomes assay). |
| Sprague-Dawley rats (2 to 21 days) | LTP induction in hippocampal slices | Hippocampal CA1 region | NMDA EPSCs recordings | Milliseconds to seconds | Rapid synaptic shift from GluN2B-NMDAR to GluN2A-NMDAR in young, but not in adult, animals, after LTP induction. | [31] |
| Adult Sprague-Dawley rats | LTP in vivo in the dentate gyrus | Dentate gyrus | WB of synaptoneurosomes, biotin-tagged synaptic surface extracts and PSD fractions | 20 h and 2 weeks after LTP induction | Increase of GluN1 in the surface-membrane fraction and synaptoneurosomes, but not in PSD fractions, at 48 h post LTP induction.
GluN1 levels in surface extracts returning to baseline at 2 weeks post stimulation. | [30] |
| Sprague-Dawley rat embryos (E18) | Glycine stimulation in hippocampal neuron cultures (21–24 DIV) | Hippocampus | (i) WB of homogenates from biotinylated primary cultures
(ii) IF of primary cultures | 30 min after glycine stimulation | Enhanced surface expression of GluN1 and GluN2A after stimulation, without change in GluN2B.
Glycine also increased total GluN1 and GluN2A protein levels. | [37] |
| Sprague-Dawley rat embryos (E18) | NMDA stimulation in hippocampal neuron cultures (21–24 DIV) | Hippocampus | (i) WB of homogenates from primary cultures
(ii) IF of primary cultures | From immediately 30 min after stimulation | Enhanced surface expression of GluN1 and GluN2A after stimulation, without change in GluN2B.
NMDA also increased total GluN1 and GluN2A protein levels. | [36] |
| Wistar rats (P 42–60) | LTP induction in hippocampal slices | Hippocampus | WB of whole-slice homogenates | 0, 30, and 70 min after LTP induction | Increase of GluN1 and GluN2A, but not GluN2B, at 70 min after LTP induction. | [34] |
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