Proteolytic Remodeling of Perineuronal Nets: Effects on Synaptic Plasticity and Neuronal Population Dynamics

Figure 1

Colocalization of perineuronal nets with parvalbumin-expressing neurons shown in (a)–(c) is a region of interest from the prefrontal cortex of a PVCretdTomato mouse. Mice were generated through crosses between PV-Cre (B6.129P2-Pvalbtm1(cre)Arbr/J; JAX #008069) driver and tdTomato (Ai14; JAX #07914) reporter animals and bred so as to avoid the confound of ectopic expression [19]. In (e)–(g), closeup views are shown from the cells noted by asterisks in (b)–(d). This 30 μm slice was incubated with fluorescein-labeled (green) Wisteria floribunda lectin (WFA) (1 : 1000, Vector Laboratories, FL-1351). WFA labels PNNs, which can be observed along the soma and proximal dendrites of several PV neurons shown in low-power views and appreciated along the same regions in the high-power image of one neuron (g) (scale bar: (a)–(c), 50 μm; (e)–(g), 20 μm). Shown in (d) is representative PNN staining in the murine sensory cortex. The schematic, in which individual PNN components are highlighted, is shown in (h).