Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration

Figure 2

Myelin content within the optic nerve. (a–d) MBP labeling (green) two weeks after optic nerve injury. (a and c) Optic nerve from the Crush-only group exhibiting large amount of MBP staining compared to (b and c) optic nerve from the Regenerating group. (e) Quantification of MBP levels. (f, g) EM images showing myelin debris in both groups. Arrows point to myelin debris, whereas arrowheads indicate degenerating nerve fibers; (h–j) CC1 staining (green) two weeks after optic nerve injury; (h) optic nerve from the Crush-only group exhibiting a reduced CC1 staining; (i, j) optic nerve image and quantitative analysis showing a higher density of CC1 staining in the Regenerating group. per group. Scale bars: (a, b) 50 μm; (c, d) 25 μm; (f, g) 2 μm; (h, i) 25 μm. Error bars in (e) and (j) show SEM. .

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