Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration

Figure 3

Macrophage/microglia within the optic nerve. (a–c) Iba-1 (red) and nuclear staining (blue) two weeks after injury. (a) Optic nerve from the Crush-only group showing a reduced Iba-1 staining; (b and c) optic nerve and quantitative analysis exhibiting intense levels of Iba-1 in the Regenerating group. (d–f) Tmem (red) and nuclear (blue) staining two weeks after injury. (d) Optic nerve from the Crush-only group. (e and f) Optic nerve image and quantitative analysis presenting higher levels of Tmem density in the Regenerating group. per group. Scale bars: (a, b, d, e) 50 μm. Error bars in (c) and (f) show SEM. .

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