Motor Improvement of Skilled Forelimb Use Induced by Treatment with Growth Hormone and Rehabilitation Is Dependent on the Onset of the Treatment after Cortical Ablation

Figure 4

(a) Immunoblot images of nestin and actin expression in the motor cortex of the undamaged hemisphere, at 51 days postablation. Results show nestin and actin expression in homogenates of motor cortex samples from animals that were under different GH treatments (LGH7, LGH14, and LGH35) or controls (CV, LV14, and LV7/35). In each immunoblot band, either nestin or actin is identified with its corresponding GH-treated animal or control, and it comes from one animal in each case. Nestin and actin molecular weight, kilodaltons (kDa), is indicated. (b) Densitometric analysis of immunoblot images of nestin and actin displayed in (a). Arbitrary units (A.U.) of densitometry. Blue points represent the values (densitometric arbitrary units from immunoblot bands) of nestin and actin expression in each motor cortex sample that comes from one animal. Red underlined ellipse identifies samples from animals treated with GH, and green underlined ellipse identifies samples from control animals.

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