(a) Data in red show average counts of c-fos-labeled neurons for subjects who received neither the 4-week sound exposure from birth stimulus nor subsequent 2 kHz tone stimulation (199 samples from 5 subjects). Data for subjects having the neonatal exposure and then no additional sound stimulation are plotted in blue (249 samples from 5 subjects). Error bars show SEM. There is no significant difference in cell count in the estimated 2 kHz region (b) or over the entire region of interest (ROI; c). In (a), the “No Sound Stim” control data (red) has been reproduced from a previously published methods paper ([26
]; fig. 3) with permission (CC BY-NC-ND license, under Crown Copyright).