|
| Antigen | Species and type, dilution | Supplier, code | Structures labeled by primary antibodies | References |
|
| Choline acetyltransferase | Goat polyclonal, 1 : 500 | Chemicon/Millipore, Schwalbach, Germany, AB144P | Cholinergic cells, axons and axon terminals, large perisomatic terminals on motoneurons | Hellström et al. [44], Nagy et al. [45], Wilson et al. [46] |
| Iba1 (ionized calcium binding adaptor molecule 1) | Rabbit polyclonal, 1 : 1500 | Wako Chemicals, Neuss, Germany, 019-19741 | Microglial cells | Imai et al. [64], Ito et al. [65] |
| VGAT (vesicular GABA transporter) | Mouse monoclonal, 1 : 500 | Synaptic Systems, Gottingen, Germany, 131 011 | Inhibitory (GABAergic and glycinergic) axon terminals | Chaudhry et al. [66], McIntire et al. [67], Wojcik et al. [68] |
| VGLUT1 (vesicular glutamate transporter 1) | Rabbit polyclonal, 1 : 1000 | Synaptic Systems, 135 303 | Excitatory (glutamatergic) axon terminals of primary (Ia) afferents | Alvarez et al. [69], Oliveira et al. [70], Rotterman et al. [10] |
| VGLUT2 (vesicular glutamate transporter 2) | Rabbit polyclonal, 1 : 1000 | Synaptic Systems, 135 403 | Excitatory (glutamatergic) axon terminals of spinal cord interneurons | Alvarez et al. [69], Oliveira et al. [70] |
| SNAP-25 BoTox-A cleaved | Mouse monoclonal (4F3-2C1), 1:200 | MyBioSource, San Diego, CA, USA, MBS350064 | Synaptic terminals containing SNAP-25 (synaptosomal-associated protein 25) cleaved by botulinum toxin A | Manufacturer’s data sheet, Rheaume et al. [32] |
| Goat IgG | Cy3-conjugated donkey polyclonal, 1 : 200 | Jackson ImmunoResearch Europe, Suffolk, UK, 705-165-003 | | |
| Mouse IgG | Cy3-conjugated goat polyclonal, 1 : 200 | Jackson ImmunoResearch, 115-165-003 | | |
| Rabbit IgG | Cy3-conjugated goat polyclonal, 1 : 200 | Jackson ImmunoResearch, 111-165-003 | | |
|
