Exposure to Patterned Auditory Stimuli during Acute Stress Prevents Despair-Like Behavior in Adult Mice That Were Previously Housed in an Enriched Environment in Combination with Auditory Stimuli

Figure 4

Effects of preexposure to patterned auditory stimuli on the number of doublecortin cells. (a) Representative images of coronal sections within the dentate gyrus showing DCX-labeled cells along the inner part of the granular cell layer (GCL). Images also show the subgranular zone (SGZ) of the dentate gyrus, the molecular layer (ML), and the hilus. (b) High-power image exhibits the presence of DCX-labeled cells in the dentate gyrus. Scale bars in (a, b) are equal to 150 and 50 μm, respectively. (c) Number of DCX cells in each group. Data represent the mean ± standard error of the mean (SEM). Housing and PAS during housing exert significant main effects. Asterisks in (c) indicate () in ENR mice compared to normal housing mice. Crosses indicate () in groups of mice that were not exposed to PAS during housing compared to mice housed in the presence of PAS. per group.

(a) Doublecortin

(b) High-power images of doublecortin

(c) Cellular quantification