CRMP2 and CRMP4 Are Differentially Required for Axon Guidance and Growth in Zebrafish Retinal Neurons

Figure 4

CRMP4 and Nrp1a synergize in retinal axon guidance. A low dose of either CRMP4 or Nrp1a induce a small percentage of ipsilateral misprojections. A half dose of either CRMP4 MOs or Nrp1a MOs was injected singly or in combination. (a, b) Representative images of knocking down effects by a half dose of Nrp1a MOs or Nrp1a and CRMP4 in combination. Some retinal axons fail to cross the midline and misproject ipsilaterally (arrows). (c) The combination of the two morpholinos induce a significantly higher percentage of ipsilateral misprojections than simply adding up the misprojections caused by the two half doses (Fisher’s exact test, ). The doses of MOs are labeled under each column. The numbers in each column indicate the amount of eyes.

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