Pharmacological Modulation of Three Modalities of CA1 Hippocampal Long-Term Potentiation in the Ts65Dn Mouse Model of Down Syndrome
The effects of memantine on L-LTP. (a) Comparison of the effects of memantine (1, 3, and 10 μM) on 4xHFS-induced L-LTP in euploid control-derived slices and (b) Ts65Dn-derived slices. Magnification of the final 10 minutes of recording. Colored lines represent the mean fEPSP slopes calculated for the last 10 minutes of recording. (c) Summary graph of euploid littermate control L-LTP data and (d) Ts65Dn L-LTP data (mean fEPSP slope during the last 10 min for each recording condition). Memantine at 3 and 10 μM blocked the induction of L-LTP in euploid control slices and had no effect on Ts65Dn-derived slices. (e) Normalized data shows a greater inhibited L-LTP in euploid control slices at 3 and 10 μM when compared to Ts65Dn slices. ,, and are represented by ,,, respectively. Number of slices (animals) for control (without mem (), mem 1 μm (), mem 3 μm (), and mem 10 μm ()) and Ts65Dn (without mem (), mem 1 μm (), mem 3 μm , and mem 10 μm ()). Error bars represent SEM. Arrows indicates LTP induction (4xHFS; with a 5 min intertrain intervals), representative traces show synaptic response during baseline (1) and at end of recording (2). Scale bars represent 1 mV (horizontal) and 10 ms (vertical).