Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain

Figure 5

Immunohistochemical staining of TSPO expression in the spinal dorsal horn following CCI. (a) Representative experiments show the change in TSPO in the ipsilateral spinal dorsal horn from sham-operated rats and rats that received the CCI operation, as well as TSPO expression on postoperative day 9 in the ipsilateral spinal dorsal horn from CCI-operated rats after repeated treatment with koumine or saline from postoperative day 3 to day 9. Scale bars = 200 μm. (b) The fluorescence density of the TSPO for the ipsilateral spinal dorsal horn from sham-operated rats and rats that received the CCI operation (). , compared with the saline or sham group, using two-way ANOVA followed by Bonferroni or Dunnett’s T3 test for each group. (c) The fluorescence density of the TSPO expression on postoperative day 9 in the ipsilateral spinal dorsal horn from CCI-operated rats after repeated treatment with koumine or saline from postoperative day 3 to day 9 (). , , compared with the saline or sham group, using one-way ANOVA followed by Bonferroni or Dunnett’s T3 test for each group.

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