Ethanol Exposure Induces Microglia Activation and Neuroinflammation through TLR4 Activation and SENP6 Modulation in the Adolescent Rat Hippocampus

Figure 5

The modulatory effect of SENP6 in ethanol-induced neuroinflammation. (a) The statistical analyses of SENP6 levels in cultured microglial cells treated with different doses of ethanol (0, 10, 30, 50, and 100 mM) for 24 h. (b) The transfection efficiency by comparing the SENP6 level of the SENP6 and siSENP6 groups to Scr group. (c–g) The levels of IkB-α, p-NF-κB-p65, TNF-α, IL-1β, and IL-6 in microglial cells of the Scr group with nonethanol treatment and of Scr, SENP6, and siSENP6 groups after treatment with ethanol at100mM for 24 h. Scr-Con = scrambled sequence group with nonethanol treatment; Scr-E = scrambled sequence group with 100 mM ethanol treatment; SENP6-E = overexpressed SENP6 group with 100 mM ethanol treatment; siSENP6-E = siRNA against SENP6 group with 100 mM ethanol treatment. Quantitative analyses were normalized to β-actin (for SENP6), GADPH (for IkB-α), or Lamin A/C (for p-NF-κB-p65) for WB. And cytokine concentrations were normalized to the total protein content. Data are presented as the . , , .

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