Novelty and Novel Objects Increase c-Fos Immunoreactivity in Mossy Cells in the Mouse Dentate Gyrus

Figure 4

Quantitative differences in hilar c-fos+ cells between control mice and mice exposed to novel objects. (a, 1) The mean number of hilar c-fos+ cells is listed according to their septotemporal location. A RMANOVA showed a significant effect of the behavioral task (control vs. novel object: ; ) and a significant effect of the septotemporal location (relatively ventral or dorsal: ; ) but no interaction of factors (; ). (a, 2) The values for hilar c-fos+ cells in the 4 most ventral and 4 most dorsal sections were pooled and were listed as ventral and dorsal, respectively. The total (ventral+dorsal) is listed as well. A two-way ANOVA showed a significant effect of the behavioral task (control vs. novel object: ; ). There was a significant effect of septotemporal location (; ) with the novel object group showing significantly more ventral than dorsal c-fos protein expression (Tukey’s post hoc test, ) and no interaction of factors (; ). Totals were significantly different (Student’s -test; ; ; ). (b, 1) The mean number of c-fos+ cells in the GCL is listed according to their septotemporal location (ventral or dorsal). A RMANOVA showed no significant effect of the behavioral task (control vs. novel object: ; ) and a significant effect of the septotemporal location (; ). There was no interaction of factors (; ). (b, 2) The values for GCL c-fos+ cells in the 4 most ventral and 4 most dorsal sections are pooled and are shown; average total values (ventral+dorsal) are also presented. A two-way ANOVA showed no significant effect of the behavioral task (; ). There were more dorsal than ventral c-fos+ GCs (; ; Tukey’s post hoc test, ) and no interaction of factors (; ). Totals were not significantly different (Student’s -test; ; ; ).