Intramuscular Injection of Adenoassociated Virus Encoding Human Neurotrophic Factor 3 and Exercise Intervention Contribute to Reduce Spasms after Spinal Cord Injury

Figure 6

The number of ventral horn motor neurons increased, and the average size increased in the combined treatment group, but there was no significant difference in SYP. (a) Schematic diagram of the spinal cord layer, with the white rectangular box indicating the position of the anterior horn Rexed’s lamina IX. (b) Immunofluorescence staining of cholinergic motor neurons ChAT (red) and SYP (green) at low magnification. (c) Expression of ChAT (red) and SYP (green) in ventral horn motor neurons in each group. Scale bar: 20 μm. (d) Bar graph showing changes in the number of motor neurons. (e) Bar graph showing changes in the size of motor neurons. (f) Bar graph showing the average number of SYP puncta in each spinal motor neuron. Data are expressed as the mean ± S.E.M. One-way ANOVA was used, and each experimental group was compared with the control group. , . ns: nonsignificant.

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