Intramuscular Injection of Adenoassociated Virus Encoding Human Neurotrophic Factor 3 and Exercise Intervention Contribute to Reduce Spasms after Spinal Cord Injury

Figure 8

Compared with the sham surgery-treated group, the number of GABAergic interneurons in the central spinal cord gray matter (Rexed’s lamina VII) was significantly increased but the size did not change. (a) Schematic diagram of the spinal layer, with the white rectangular box indicating the position of the VII layer. Representative immunofluorescence staining of GAD65-positive GABAergic interneurons (green) in each group. Scale bar: 50 μm. (b) Bar graph showing changes in the number of GABAergic interneurons in each group. Number of cells counted per field (307 μm × 307 μm). (c) Bar graph showing changes in the size of GABA interneurons in each group. Data are expressed as the mean ± S.E.M. One-way ANOVA was used, and each experimental group was compared with the control group. . ns: nonsignificant.

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