Memantine Differentially Regulates Tau Phosphorylation Induced by Chronic Restraint Stress of Varying Duration in Mice

Figure 7

Modulation of tau kinases and phosphatase by memantine after 28 consecutive days of chronic restraint stress (CRS). (a) Frontal cortex lysates from the four mouse groups were immunoblotted with antibodies against p-AKT (Ser473), AKT, p-GSK3β (Ser9), GSK3β, p-ERK (Thr202/Tyr204), ERK, p-JNK (Thr183/Tyr185), JNK, p-P38 MAPK (Thr180/Tyr182), P38 MAPK, and CDK5; memantine increased the levels of p-AKT, p-ERK, and p-JNK, while reducing those of p-GSK3β. (b–g) Densitometric quantification of the bands in (a). (h) Western blotting showed that 28-day CRS induced increase of DM-PP2Ac and pY307-PP2Ac, and memantine enhanced the levels of DM-PP2Ac and pY307-PP2Ac. (i–k) Densitometric quantification of the bands in (h). The results are shown as means ± SEM ( in each group); β-actin and GAPDH were used as loading controls. ; ; . Con: control.

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