Changes in the Fluorescence Tracking of NaV1.6 Protein Expression in a BTBR T+Itpr3tf/J Autistic Mouse Model

Figure 3

Ankyrin-G expression in the PFC of the BTBR mouse model. (a, b) High-resolution confocal images acquired from the PFC of C57BL/6J and BTBR: ankyrin-G (grey and red) and NeuN (green). (c) The ROI mask used to quantify ankyrin-G. (d) The quantification of ankyrin-G immunofluorescence. mice per group. (e) Representative high-resolution confocal images of AIS in C57BL/6J and BTBR mice. (f) The AIS tracking analysis of ankyrin-G in C57BL/6J and BTBR mice. mice per group. (g) Ankyrin-G protein levels in PFC lysates from C57BL/6J and BTBR mice and the Western blot bands. The protein expression was normalized with β-actin. -5 mice per group. Data represent ; statistical differences were assessed using Student’s -test (). Scale bars represent 20 μm in (b) and 10 μm in (e).