Neuronal Pentraxin 2 Binds PNNs and Enhances PNN Formation

Figure 2

Nptx2 is expressed in the cerebral cortex. (a) These images were taken from the Gene Expression Nervous System Atlas. Bacterial Artificial Chromosome engineering was applied to express an EGFP gene upstream of the Nptx2 gene. High expression of Nptx2 was found in the cerebral cortex of mice at P7. Low expression was found in the cerebral cortex of adult mice, at P42. Low to no expression was found in the cerebral cortex of mice at E15.5. These findings indicate that expression of Nptx2 is high during perineuronal net development, after which it is reduced. The black arrows indicate neurons which express Nptx2 (). (b) These images were taken from the Allen Brain Atlas. The images were produced with in situ hybridization for Nptx2 on male mice at p56. They show that expression of Nptx2 is present in adult mice in the cortex: Nptx2-RP_051214_01_H10–coronal, © 2010 Allen Institute for Brain Science, Allen Human Brain Atlas, available from http://human.brain-map.org. (c) Violin representation of scRNA-Seq in 14236 single cells from the visual sensory primary cerebral cortex in mice 56 days old. Data are from the Allen Institute (file reference VISp_14236_20180912). On the top 13 subclasses are 4 GABAergic (Lamp5, Vip, Sst, and Pvalb), 8 glutamatergic (layer 2/3 IT IntraTelencephalic excitatory neurons, layer 4 excitatory neurons, layer 5 IT, layer 6 IT, layer 5 PT Pyramidal Tract excitatory neurons, NP Near Projecting excitatory neurons, layer 6 CT corticothalamic excitatory neurons, layer 6b excitatory neurons), and 1 astrocyte in various colors according to the code color of Tasic et al. [45]. On vertical left genes detected; Gad2 marker for interneurons; Slc17a7 marker for excitatory neurons; Gaj1 marker for astrocytes; Lamp (lysosomal-associated membrane protein family, member 5), Vip (vasoactive intestinal polypeptide), Sst (Somatostatin), Pvalb (Parvalbumin), four markers delineating four subclasses of interneurons: Sema3a, Sema3e, Nptx1, and Nptx2. Median values are black dots, and values within rows are normalized between 0 and the maximum expression value for each gene (right edge of each row) and displayed on a log10 scale.

(a)

(b)

(c)