iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression

Zhang, Jialing; Zhang, Zhinan; Zhang, Jiping; Zhong, Zheng; Yao, Zengyu; Qu, Shanshan; Huang, Yong

doi:https://doi.org/10.1155/2019/7492306

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Research Article | Open Access

Volume 2019 | Article ID 7492306 | https://doi.org/10.1155/2019/7492306

iTRAQ-Based Protein Profiling in CUMS Rats Provides Insights into Hippocampal Ribosome Lesion and Ras Protein Changes Underlying Synaptic Plasticity in Depression

Jialing Zhang,¹Zhinan Zhang,²Jiping Zhang,²Zheng Zhong,³Zengyu Yao,²Shanshan Qu,²and Yong Huang²

Academic Editor: Grzegorz Hess

Received16 Nov 2018

Revised20 Feb 2019

Accepted26 Feb 2019

Published02 May 2019

Abstract

Hippocampal atrophy is one of the key changes in the brain implicated in the biology of depression. However, the precise molecular mechanism remains poorly understood due to a lack of biomarkers. In this research, we used behavioral experiments to evaluate anxiety and anhedonia levels in depressed rats using chronic unpredictable mild stress (CUMS) modeling. We also used isobaric tag for relative and absolute quantitation (iTRAQ) to identify the differentially expressed hippocampal proteins between depressed and normal rats. Bioinformatics analyses were also performed for a better understanding. The results showed that CUMS rats had higher anxiety and anhedonia levels than control rats, along with hippocampal lesions. Through iTRAQ and bioinformatics analyses, we found that ribosome proteins were significantly downregulated and Ras proteins exhibited a mixed change in the hippocampus of depressed rats. These findings suggest that the expression of hippocampal ribosome lesions and Ras proteins is significantly different in depressed rats than in control rats, providing new insights into the neurobiology of depression.

1. Introduction

Approximately 50% of suicide victims worldwide suffer from depression or another mood disorder, which makes depression one of the leading causes of disease burden [1, 2]. Efforts have been made to understand the biology of depression. Several theories have been raised regarding the issue. One of the dominant theories is the monoamine hypothesis, which postulates that a deficit of certain neurotransmitters is responsible for depression [3]. The monoamine hypothesis is based on the observation that many antidepressants increase neurotransmitters at synaptic levels [4]. However, the limitation of the monoamine hypothesis is revealed by the 1-2-week therapeutic lag of these antidepressants, such as selective serotonin reuptake inhibitors (SSRIs) [5]. Additionally, researchers have found that serotonin knockout animals do not have typical depressive behaviors [6]. Therefore, the monoamine hypothesis might oversimplify the problem.

The inflammation theory has also been raised, stressing the role of inflammation in depression. Various reviews have found that depression patients have high levels of inflammatory cytokines, such as interleukin- (IL-) 1β, IL-6, and tumor necrosis factor- (TNF-) α [7]. When exposed to stress, the translation of inflammatory cytokines is activated. Overexpressed inflammatory cytokines travel through the blood-brain barrier (BBB) or are released by microglia and influence brain function [8]. Studies have also demonstrated that anti-inflammatory therapies can alleviate the symptoms [9].

Among all these theories, stressor exposure has proven to be the most robust factor associated with the development of depression [10]. In response to stressors, the hypothalamic-pituitary-adrenal (HPA) axis is activated. Long-term exposure causes HPA axis dysfunction and high levels of glucocorticoids, which result in cell loss and compromise neurogenesis in the hippocampus [11]. Therefore, atrophy of the hippocampus is considered one of the main features of the depressed brain, which has been repeatedly observed in humans and rodents [12, 13].

Recent studies have also called attention to the role of disrupted synaptic plasticity (the ability of synapses to strengthen or weaken over time) in depression. Studies have demonstrated that synapse number is significantly reduced in certain crucial brain regions, such as the prefrontal cortex (PFC) and hippocampus [14]. In addition, depressed animals have impaired hippocampal long-term potentiation (LTP), which is a pattern of synaptic activity in which a long-lasting increase in synaptic strength is observed [15].

Proteomic technologies are the ideal techniques for the detection and investigation of biomarker candidates, owing to the high sensitivity and analytical performance that can be achieved and the ability to generate large datasets through the identification of large and ever-increasing numbers of proteins [16–18]. Isobaric tag for relative and absolute quantitation (iTRAQ) is a proteomic approach that can determine the amount of proteins from different sources in a single experiment [19]. This technology has been used to outline the proteomic profiles of cancers [20]. Currently, a number of biomarkers for bladder cancer have been detected in urine and tissue using this technique [21].

In this study, we identified differentially expressed proteins in the depressed and normal hippocampus using iTRAQ. Regarding the abundance of the proteins and their biological information, bioinformatics analyses were performed to identify possible proteins underlying the biology of depression.

2. Methods

2.1. Animals

A total of 25 Wistar rats (males; weight 180-200 g; Southern Medical University Experimental Animal Center) were acclimated to an SPF facility (temperature °C, humidity 50%-60%) at Southern Medical University, China. Rats were housed individually with a constant 12 h light/dark cycle (lights on/off at 07:00/19:00) unless otherwise noted. Rats were bred normally for at least 6 days for adaption before the CUMS paradigm. Food and water were available ad libitum. Rats were randomly assigned into 2 groups: control () and CUMS ().

2.2. CUMS Paradigm

CUMS rats underwent a 21-day chronic unpredictable mild stress procedure. On each of the 21 consecutive days, rats were exposed to a random stressor (Figure 1(a)). These stressors included water deprivation (24 h), food deprivation (24 h), wet bedding (24 h), light-dark reversal (24 h), stroboscopic lighting (12 h), immobilization (2 h), cold swim (4°C, 5 min), warm swim (45°C, 5 min), level shaking (5 min), and tail clamping (3 min).

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2.3. Behavioral Experiments

The sucrose preference test (SPT) was used to assess anhedonia. After a 2-day habituation phase, rats were housed singly for 24 h without any food or water. Then, the rats were presented with two identical bottles containing either sucrose solution (1%) or pure water for 1 h. The sucrose preference rate was calculated as the amount of sucrose solution consumed relative to the total fluid consumed.

One day after the SPT, an open-field test (OFT) was performed to evaluate anxiety. The open-field arena ( cm) was equally divided into 25 square areas. The 9 grids in the center were defined as the central region. Rats were individually placed into the arena for 5 min. Distance travelled and time spent in the central zone were analyzed using video cameras with associated software (Smart 2.0).

The behavioral experiments and weighing of the animals were performed before and after the CUMS paradigm (Figure 1(a)).

2.4. Hippocampus Tissue Acquisition

After the behavioral experiments, rats were exposed to 25% pentobarbital sodium (50 mg/kg, intraperitoneal injection) and subsequently decapitated. Brains were instantly dissected, and all attached tissues were removed. Hippocampus tissues were separated, rinsed with phosphate-buffered saline (PBS), immediately frozen in liquid nitrogen, and stored at -80°C until analyses (3 rats/group). For hematoxylin and eosin (H&E) staining, cornu ammonis (CA) 1 was separated, fixed in 4% paraformaldehyde, embedded in paraffin, sliced, deparaffinized, and stained for routine H&E staining and histological examination (6 rats/group).

2.5. Protein Preparation, iTRAQ Isobaric Labeling, and SCX Separation

Hippocampus tissues were ground into powder in liquid nitrogen using lysis buffer (Roche). Then, the samples were ultrasonically disrupted on ice. Supernatants were collected after centrifugation (10,000g, 30 min, 4°C), and protein concentrations were determined using an enhanced BCA (bicinchoninic acid) Protein Assay Kit (P0010; Beyotime Biotechnology Ltd., Beijing, China), according to the manufacturer’s instructions. The protein samples (200 μg) were mixed with dl-dithiothreitol, alkylated with iodoacetamide, and then treated with trypsin (protein-trypsin : 1, 12 h).

Protein peptides (100 μg) from each group were labeled using an iTRAQ Reagent-8plex Multiplex Kit (AB SCIEX, Framingham, MA, USA). The samples were labeled as 113 (control 1), 114 (control 2), 115 (CUMS 1), 116 (CUMS 2), and 117 (CUMS 3). The labeled samples were pooled and further fractionated offline using the ÄKTApurifier 100 (GE Healthcare Life Sciences) with a strong cation exchange column (PolySULFOETHYL A™; PolyLC Inc., Columbia, MD, USA). The retained peptides were eluted with buffer A (10 mM KH₂PO₄ in 25% ACN (acetonitrile), pH 3.0) and buffer B (10 mM KH₂PO₄ and 500 mM KCl in 25% ACN, pH 3.0) with a flow rate of 0.7 ml/min.

2.6. LC-MS/MS Analysis

Eluted fractions were lyophilized using a centrifugal speed vacuum concentrator (CentriVap® Complete Vacuum Concentrator; Labconco, Kansas City, MO, USA) and dissolved in formic acid (5 μl, 0.5%). Equivalent amounts of peptides from each fraction were mixed and then subjected to reversed-phase nanoflow LC-MS/MS analysis using a high-performance liquid chromatography (HPLC) system (EASY-nLC™, Thermo Fisher Scientific) connected to a hybrid quadrupole/time-of-flight mass spectrometer equipped with a nanoelectrospray ion source. The peptides were separated on a C18 analytical reversed-phase column with mixtures of solution A (0.1% formic acid in water) and solution B (0.1% formic acid in ACN). A full MS scan was conducted using a Q Exactive™ mass spectrometer (Thermo Fisher Scientific) with a flow rate of 600 nl/min. Mass spectrometry was then performed using a mass spectrometer (Q Exactive HF, Thermo Fisher Scientific).

2.7. Protein Identification and Quantification

Raw MS/MS data were searched against the UniProt database (last modified on April 22, 2017) using Mascot 2.2 and Proteome Discoverer™ 1.4 software (Thermo Fisher Scientific). A peptide false discovery rate was used as the identification standard. Protein quantification was based on the total intensity of the assigned peptides. The average of labeled sample mixes was used as a reference and was based on the weighted average of the intensity of the reported ions in each peptide identified. The final protein ratios were normalized to the median average protein content of the 8-plex samples. A 1.2-fold cutoff was set to identify upregulated and downregulated proteins.

2.8. Bioinformatics Analysis

The functional enrichment analysis of significantly changed proteins was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis with the online software DAVID (https://david.ncifcrf.gov/). Corrected values < 0.1 were considered significantly enriched. Protein-protein interaction (PPI) networks were retrieved from STRING (https://string-db.org/) using Cytoscape software (Version 3.6.1, https://cytoscape.org/). The Markov cluster algorithm (MCL) was then performed to determine topological clusters of the network using Cytoscape software.

2.9. Statistical Analysis

All data are expressed as the . Differences in behavioral results and differentially expressed proteins were evaluated using Student’s t-test. All statistical analyses were carried out using SPSS software (version 20.0, SPSS Inc., USA). was considered statistically significant.

3. Results

3.1. Stressors Cause Behavioral and Hippocampal Abnormalities

We used a 21-day chronic unpredictable mild stress (CUMS) paradigm to model human depression in rats. Animals were exposed to chronic unpredictable mild stressors for 21 days (Figure 1(a)). To measure appetite, anxiety, and anhedonia levels, body weight, open-field test (OFT), and sucrose preference test (SPT) were used, respectively.

The CUMS paradigm resulted in a lower weight in CUMS rats than in control rats (Figure 1(b)). CUMS rats also had decreased time spent in the central zone and distance travelled in the OFT, indicating increased anxiety (Figures 1(c) and 1(d)). In the SPT, CUMS rats had a lower sucrose preference rate than control rats, indicating anhedonia (Figure 1(e)).

The hippocampus is one of the important brain regions involved in the biology of depression. Hippocampus tissue was obtained for histology examination. H&E staining showed that control rats had a thick hippocampal pyramidal cell layer as well as densely, closely, and regularly arranged cells in CA1. In contrast, CUMS rats had a thin pyramidal cell layer, widened intercellular spaces, and irregularly and loosely arranged cells. Therefore, tissue damage and cell apoptosis occurred in the hippocampus of CUMS rats (Figures 1(f) and 1(g)).

3.2. Changes in the CUMS Hippocampal Proteomic Profile

To further understand the mechanism of depression, we used iTRAQ to identify differentially expressed hippocampal proteins between groups. Based on the iTRAQ-LC-MS/MS analysis results, a total of 3511 proteins and 18,381 peptides were identified (peptide false discovery rate ). Most of the identified proteins (75.39%) had molecular weights in the range of 10-80 kDa (Figure 2(a)). Approximately 60.84% of the identified proteins had more than 2 peptides (Figure 2(b)).

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Fifty-two quantified proteins with and an expression change of higher than 1.50-fold or lower than 0.67-fold between the CUMS and control groups were manually selected (Table 1). Thirty differentially expressed proteins were upregulated, and 22 were downregulated after the CUSM paradigm.

3.3. Functional Annotation Enrichment of the Differentially Expressed Proteins

To understand the biological meaning behind the large list of proteins differentially expressed between groups and the underlying mechanism of depression, differently expressed proteins were subjected to enrichment analysis using the DAVID website. The identified enriched biological themes included biological process, molecular function, cellular component, and KEGG pathway. Enrichment analyses were performed on all differentially expressed proteins and then on upregulated and downregulated proteins separately for better understanding.

For all the differentially expressed proteins, the enrichment analysis results showed that, in terms of biological process, most of the differentially expressed proteins were involved in response to peptide hormone (7.69%, ), regulation of cell morphogenesis (5.77%, ), negative regulation of NF-kappaB transcription factor activity (5.77%, ), response to prostaglandin F (3.85%, ), isoprenoid biosynthetic process (3.85%, ), positive regulation of protein autophosphorylation (3.85%, ), multicellular organism aging (3.85%, ), response to metal ion (3.85%, ), and Rap protein signal transduction (3.85%, ). Regarding molecular function, most of the differentially expressed proteins were annotated as being associated with calcium ion binding (11.54%, ). In terms of cellular components, most of the differentially expressed proteins were predicted to be in the endomembrane system (5.77%, ). For KEGG pathways, most of the differentially expressed proteins were involved in terpenoid backbone biosynthesis (3.85%, ) and protein export (3.85%, ) (Figure 3(a)).

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For upregulated proteins, only biological process enrichment was found, and most of the upregulated proteins were involved in the positive regulation of protein autophosphorylation (6.67%, ), Rap protein signal transduction (6.67%, ), protein K48-linked ubiquitination (6.67%, ), protein K63-linked ubiquitination (6.67%, ), and microvillus assembly (6.67%, ) (Figure 3(b)).

For downregulated proteins, enrichment analyses results showed that, in terms of biological process, most of the downregulated proteins were involved in the regulation of cell morphogenesis (9.09%, ), inner ear development (9.09%, ), lung development (9.09%, ), and response to cAMP (9.09%, ). Regarding molecular function, most of the downregulated proteins were annotated as being associated with NF-kappaB binding (9.09%, ), the structural constituents of ribosomes (18.18%, ), and poly(A) RNA binding (27.27%, ). In terms of the cellular component, most of the downregulated proteins were predicted to be in the endomembrane system (13.64%, ) and membrane (40.91%, ) (Figure 3(c)). KEGG pathway enrichment was not found in downregulated proteins.

3.4. Protein-Protein Interaction Network of the Differentially Expressed Proteins

First, we retrieved the interaction network of all 52 differentially expressed proteins. MCL was performed to explore the strong connections between groups of nodes. Examining the main connected component of the network, we immediately found that there were 3 clusters of proteins: Psmb4, Mrpl46, Dars2, Ranbp6, Rpl14, Agfg2, Itch, Mrps5, RGD1560248, Timm10b, RGD1561333, and Anp32b; Dnajc27, Chp, Arhgap12, Neo1, Tbc1d13, Rac3, Rap2a, Rap2b, Sparc, Syne1, Fmod, and Fam136a; and Stx5, Manf, Th, Arcn1, Sec63, Spcs3, and Dynll1 (Figure 4(a)).

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To further understand the network, we also examined upregulated and downregulated protein networks separately. MCL clustering was also performed. Examining the main connected component of the network of upregulated proteins, we found 2 clusters of proteins, one of which consisted of Rap2b, Rap2a, Chp, Ap1s2, Fmod, and Syne1 (cluster 1). Both Rap2b and Rap2a belong to the family of Ras-related proteins, also known as Rap GTP-binding protein, one of the subfamilies of the Ras superfamily (Table 2). Members of this superfamily appear to regulate a diverse array of cellular events, including cell growth control, cytoskeletal reorganization, and protein kinase activation.

The other cluster consisted of Th, Stx5, Dynll1, and Arcn1 (cluster 2) (Figure 4(b)). These proteins are mainly involved in vesicle structure and trafficking. For instance, Stx5 is a member of the syntaxin or t-SNARE (target-SNAP receptor) family, which plays a crucial role in synaptic vesicle docking. Notably, Th is a rate-limiting enzyme in the synthesis of catecholamines, which is the process necessary for the formation of the dopamine (DA) precursor levodopa (l-DOPA). Hence, Th plays a key role in the biosynthesis of dopamine (Table 2).

As for the network of downregulated proteins, one cluster of downregulated proteins contained Mrps5, Psmb4, Mrpl46, RGD1561333, Anp32b, Fam136a, and Rpl14 (cluster 3). These proteins are mostly ribosome translation related. For example, Mrps5, Mrpl46, and Rpl14 are all ribosomal subunit proteins, and RGD1561333 and Anp32b are both involved in translation (Table 3).

There was also a cluster of proteins, Rac3, Tbc1d13, Neo1, Arhgap12, and Dnajc27, that was predominantly downregulated (Figure 4(c)), and which was mainly relevant to the Ras superfamily of small GTP-binding proteins and subsequent signaling pathways. For example, Rac3 and Dnajc27 belong to the Rho and Rab protein families, respectively, which are subfamilies belonging to the Ras superfamily. Tbc1d13 binds to Rab GTPase (Table 3).

4. Discussion

After exposure to stressors for 21 days, the CUMS rats exhibited less time spent in the central zone, less distance travelled, and lower sucrose preference in the OFT and SPT, as well as decreased weight, indicating elevated anhedonia and anxiety levels. Hippocampus lesions were also observed. These results suggest that the depression model was successfully established. To understand the hippocampal proteomic changes underlying the mechanism of depression, we used LC-MS/MS analysis and bioinformatics analysis to identify the significantly changed proteins between the CUMS and control groups. We found GO enrichment in the GO term “Rap protein (a subfamily of Ras superfamily) signal transduction” among all differently expressed proteins and in the “structural constituent of ribosome” among downregulated proteins (Figure 3). Similarly, in the MCL cluttering analyses, some identified clusters are involved in ribosomal translation and are relevant to the Ras superfamily (Figure 4). Together, these findings suggest that hippocampal ribosome lesions and Ras protein changes underlie the mechanism of depression.

4.1. Ribosome and Depression

Ribosomes serve as the workplace of RNA translation, which makes them vital organelles for protein synthesis [22]. In the neural system, ribosomes are known to contribute to neuron development. Moreover, rapid, local activation of protein synthesis in ribosomes is required for synaptic plasticity [23]. Ribosomes not only exist in the soma of neurons but also play an important role in axons and synapses. RNA is transferred to its postsynaptic destination and subsequently translated in the postsynaptic ribosome [24]. A recent study also demonstrated that presynaptic protein synthesis in the ribosome is essential for the long-term plasticity of neurotransmitter gamma-aminobutyric acid (GABA) release [25].

In our study, the expression of ribosome proteins was significantly decreased in the hippocampus of depressed (CUMS) rats, especially the expression of ribosomal subunit proteins Mrps5, Mrpl46, and Rpl14 (Figure 4(a)). Similar studies have also revealed ribosome lesions in depression patients and animal models [26–28]. Interestingly, both Mrps5 and Mrpl46 belong to the family of mitochondrial ribosomal proteins (MRPs).

Research has found that MRPs are evolutionarily conserved proteins that serve as metabolic and longevity regulators. MRPs play a crucial role in activating the mitochondrial unfolded protein response (UPR^mt) and therefore maintaining the balance of mitochondrial-nuclear proteins and extending lifespan [29]. Lifespan enhancers such as rapamycin and resveratrol also share this mechanism [30]. UPR^mt activation has been observed in a mouse model of depression caused by chronic restraint [31]. These studies and ours provide a new strategy in depression intervention to use rapamycin and resveratrol as supplements to alleviate depression by changing mitochondrial translation.

4.2. Ras Superfamily in Depression

The Ras superfamily is an evolutionarily conserved protein superfamily of small GTPases, including several subfamilies, such as Ras, Rho, Ran, Rab, and Arf GTPases, among which the Ras family itself is further divided into Ras, Ral, Rap, Rheb, Rad, and the recently included Rit and Miro [32]. Generally, these proteins are responsible for cell proliferation and survival [33]. Until now, the mechanism of Ras family proteins has primarily been discussed in terms of their role in tumorigenesis. However, recent studies have shown that the Ras superfamily is involved in psychiatric disorders. Ras gene mutations are found in patients suffering from psychiatric and neurodevelopmental disorders [34].

Ras proteins activate and stimulate multiple downstream effector pathways by direct interactions, such as the Raf/Mitogen-activated protein kinase kinase (MEK)/extracellular regulated protein kinase (ERK) cascade and the phosphoinositide 3-kinase (PI3K) signaling cascades [35]. These pathways mediate the control of various physiological processes. Taking PI3K signaling cascades as an example, the pathway has been found to be a necessary component in LTP [36]. Studies have also shown that these signaling cascades serve as key biochemical cascades in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking during synaptic plasticity in neurons and altered behavior [37].

Altered hippocampal synaptic plasticity is considered one of the underlying mechanisms of depression. In our research, the expression of proteins in the Ras superfamily changed significantly. The MCL clustering results showed a mixed change in these proteins, meaning some of the Ras proteins were upregulated and some downregulated. Upregulated proteins such as Rap2b and Rap2a belong to the Rap family (Figure 4(a)).

Notably, Ras and Rap proteins of the Ras subfamily function antagonistically [38]. In neurons, Ras plays a crucial role in synapse enforcement and LTP by promoting postsynaptic insertion of AMPAR. Rap weakens synapses and induces long-term depression (LTD) by increasing AMPAR internalization [39]. Our results show that typical Rap proteins, Rap2b and Rap2a, were upregulated in the CUMS hippocampus (Table 2), which indicates synaptic weakening and synaptic plasticity disturbances in depression.

On the other hand, of the downregulated proteins identified, Rac3 belongs to the Rho family and Dnajc27 belongs to the Rab family (Table 3). Specifically, Rho proteins are responsible for the morphogenesis of dendritic spines [40] and Rab for that of synaptic vesicles [41], which are both vital biological processes underlying synaptic plasticity. Therefore, we consider that Ras proteins are involved in hippocampal pathology changes by affecting hippocampal synaptic plasticity.

However, we did not conduct experiments examining the UPR^mt or synaptic plasticity of hippocampal neurons in this study. Whether these proteins are responsible for the UPR^mt and disrupted synaptic plasticity in the hippocampus is still unknown. Further research may be needed to draw a conclusion. Another possible limitation of the study is that we did not focus on a specific subfield of the hippocampus, such as the dentate gyrus (DG), CA1, CA2, CA3, or CA4. Because most of the identified proteins in this research are synapse-related, we would like to focus on the CA1 and DG in future studies. Indeed, synaptic plasticity in the CA1 is rather vulnerable in diseases, and adult neurogenesis still exists in the DG, which makes DG a subfield of high synaptic plasticity [42, 43].

Data Availability

The data used to support the findings of this study are available from the corresponding authors upon request.

Ethical Approval

This experiment was approved by the Southern Medical University Experimental Animal Ethics Committee (resolution no. L2015056).

Disclosure

Jialing Zhang and Zhinan Zhang are co-first authors.

Conflicts of Interest

The authors declare no conflict of interest in the present study.

Authors’ Contributions

Jialing Zhang, Zheng Zhong, Shanshan Qu, and Yong Huang conceived and designed the experiments. Jiping Zhang and Zengyu Yao performed the experiments. Zhinan Zhang analyzed the data.

Acknowledgments

This work was supported by the National Natural Science Foundation of China (81873359 and 81603474), the Natural Science Foundation of Guangdong Province (2016A030313522 and 2016A030310383), and the Science and Technology Program of Guangzhou (201707010041), China.

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Copyright © 2019 Jialing Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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