HMG-CoA Reductase Inhibitors Attenuate Neuronal Damage by Suppressing Oxygen Glucose Deprivation-Induced Activated Microglial Cells

Figure 1

Rosuvastatin and atorvastatin suppressed the death of neurons by blocking inflammatory stimulation in the coculture systems. (a, b) Cell viability of BV2 cells for different concentrations of rosuvastatin and atorvastatin after OGD damage was measured by MTT assays. Values shown are expressed as the as the ratio to the C group was assessed by one-way ANOVA followed by test (homogeneity of variance was determined); . vs. the C group and vs. the OGD group. (c) In the coculture systems, the BV2 cells in the upper wells were carefully placed on 6-well transwell plates and cocultured with neurons in the bottom wells. (d) Relative LDH levels in neurons cocultured with BV2 microglia were determined by LDH assays. (e, f) IL-10 expression in both BV2 cell culture supernatants and neuron culture supernatants was determined by ELISA. (b, c) Values shown are expressed as the as the ratio to the C group was assessed by one-way ANOVA followed by LSD test (homogeneity of variance was determined), except that the expression of IL-10 of BV2 between-group differences was assessed by one-way ANOVA followed by Tamhane’s test (homogeneity of variance was not determined); . vs. the C group, vs. the C group, vs. the OGD group, vs. the OGD group, and vs. the OGD group + R.

(a)

(b)

(c)

(d)

(e)

(f)