HMG-CoA Reductase Inhibitors Attenuate Neuronal Damage by Suppressing Oxygen Glucose Deprivation-Induced Activated Microglial Cells

Figure 4

Rosuvastatin and atorvastatin prevented inflammatory activity in BV2 microglia. (a) HIF1-α, Arg1, and Iba-1 were determined in BV2 cells by Western blotting using the respective antibodies. Quantified (b) HIF1-α/β-actin and (c) Arg1/Iba-1 are expressed as ratios to the C group. (d, h) TNF-α, IL-1β, COX2, pNF-κB, and pIκBα were determined in BV2 cells by Western blotting using the respective antibodies. Quantified (e) TNF-α, (f) IL-1β, (g) COX2, (i) pNF-κB, and (k) pIκBα are expressed as ratios to the C group. Values shown are expressed as the , and between-group differences were assessed by one-way ANOVA followed by LSD test (homogeneity of variance was determined), except that the expression of IL-1β between-group differences was assessed by one-way ANOVA followed by Tamhane’s test (homogeneity of variance was not determined); . vs. the C group, vs. the C group, vs. the C group, vs. the OGD group, vs. the OGD group, vs. the OGD + R group, and vs. the OGD + R group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)