Alteration of Extracellular Matrix Molecules and Perineuronal Nets in the Hippocampus of Pentylenetetrazol-Kindled Mice

Figure 3

PV, WFA, and Cat-315-positive images in the PTZ-kindled mouse hippocampus. Representative images show the laminar distribution of PV-positive neurons (a, A), WFA-positive molecules (b, B), and Cat-315-positive molecules in the mouse hippocampus of control (a–c) and PTZ-kindled mice (A–C). Representative triple immunofluorescent images show the laminar distribution of PV neurons, WFA-positive PNNs, and Cat-315-positive PNNs in the CA1 (d), CA3 (e), and DG (f) of control (upper panels) and PTZ-kindled mice (lower panels). The arrows indicate PV-positive neurons colocalized with PNN markers. The arrowheads indicate PV-positive neurons not colocalized with PNN markers. Scale bars: 500 μm in C (applies to (a)–(c), (A)–(C); 50 μm in (f) (applies to (d)–(f)). PV: parvalbumin; WFA: Wisteria floribunda agglutinin; PNN: perineuronal nets; PTZ: pentylenetetrazole.