Research Article

Functional Role of SIL1 in Neurodevelopment and Learning

Figure 2

Expression of SIL1 in cultured cortical neurons. (a) Cellular localization of SIL1 was examined in cultured cortical neurons at DIV14. SIL1 was immunostained with anti-SIL1 antibody (green), and synaptophysin or PSD95 was immunostained with anti-synaptophysin or anti-PSD95 antibody (red). Scale bar equals to 10 μm. (b) Expression of SIL1 in different subneuronal compartments of the adult mouse cortex tissue was investigated. S1 indicated extrasynaptic membrane fraction, S2 indicated Triton X-100-soluble synaptic faction, and S3 indicated Triton X-100-insoluble PSD fraction. (c, d) Interactions between SIL1 and GluN2A, PSD95, Bip, and GluN2B were examined by coimmunoprecipitation. Anti-SIL1, anti-PSD95, anti-GluN2A, and anti-GluN2B antibodies were used to precipitate the other proteins. GAPDH was used as a negative control.