Toxic Effects of 3,3-Iminodipropionitrile on Vestibular System in Adult C57BL/6J Mice In Vivo

Figure 1

A single injection of IDPN induced dose-dependent acute utricular sensory epithelium injury. The numbers of HCs, SCs, and Sox2+ HCs were counted with ImageJ software per area, two different areas in the extrastriolar region (yellow square frame) and striolar region (orange square frame) for every utricle (a1–a6). (b1–c6) showed zoomed pictures within the yellow (b1–b6) and orange (c1–c6) square rectangle tool. In controls (a1), MyoVIIa+ HCs, and Sox2+ SCs were arranged in an orderly fashion and showed no defects. MyoVIIa+ HCs became sparser dose-dependently, especially in the striolar area (b2–b6, c2–c6). No significant loss of Sox2+ SCs was observed until 5 or 6 mg IDPN/g body weight was injected (b2–b6, c2–c6). Graphs (d, e, f) show the average numbers of HCs, SCs, and Sox2+ HCs, in the extrastriolar and striolar regions per area after a single IDPN injection. Graph (g) shows the percentage of Sox2+ HCs after a single IDPN injection. The scale bars indicate 20 μm. .