Toxic Effects of 3,3-Iminodipropionitrile on Vestibular System in Adult C57BL/6J Mice In Vivo

Figure 3

The behavioral tests showed the dysfunction of the vestibular system after IDPN injection. A simple trunk curl test showed that control mice would reach towards the horizontal surface when their tails were held (a), while the mice with vestibular injury would curl towards their abdomen (b). When placed on a table, control mice would walk straight and balanced (c), while animals with vestibular impairment would walk in circles chasing their tails (d). The swim test can yield a score for general vestibular function. Mice in water will swim (e) (score 0), swim irregularly (not shown in figure, score 1), float immobile (f) (score 2), or tumble underwater (g) (score 3). Graph (h) showed the swim test scores on D7 after different concentrations of single IDPN injections which get higher with the increase in IDPN dose. However, scores showed no significant difference in the 2 mg IDPN/g body weight injected mice compared to controls. Ordinary one-way ANOVA of swim test () with Tukey’s multiple comparisons test were performed with GraphPad Prism 6.01. .