Toxic Effects of 3,3-Iminodipropionitrile on Vestibular System in Adult C57BL/6J Mice In Vivo

Figure 4

Rotating VOR test served as a sensitive measurement for evaluating the function of the vestibular system. Vestibular function was evaluated on a binocular VOG-based VFT system at 7 days (7 d), 1 month (1 m), and 3 months (3 m) after IDPN injection. A mouse was placed in the custom-built box adapted to its weight and then fixed on the rotating device (a). Yellow arrows, orange arrows, and red arrows point out the mirrors, cameras, and infrared lamps on the platform, respectively (a). Eye movements in the front mirror were recorded in real time by the side cameras as the platform rotated at 0.25, 0.5, and 1 Hz (b). Graphs (c, d, e) show the rotating VOR gain at 7 d, 1 m, and 3 m. VOR gain showed significant decrease at all three frequencies compared with control mice dose-dependently in day 7 (c) and 1 month (d). VOR gains of the 2 mg IDPN/g body weight injected mice showed no difference compared with those of the control mice after 3 months (e). Two-way ANOVA of VOR gains (all ) with Tukey’s multiple comparison test was performed with GraphPad Prism 6.01. and .

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