Toxic Effects of 3,3-Iminodipropionitrile on Vestibular System in Adult C57BL/6J Mice In Vivo

Figure 5

The limited proliferation in utricle sensory epithelium induced by IDPN injection. No MyoVIIa+/EdU+ cells were seen in any of the IDPN-treated utricles (c1–c3). EdU+/Sox2+ cells were not observed in the 4 mg IDPN/g body weight-treated utricles (e1). EdU+/Sox2+ cells appeared on the edge of the striolar area and the striolar area in the 5 and 6 mg IDPN/g body weight-treated utricles (yellow arrows) (e2, e3). Graph (f) shows the average number of EdU+/Sox2+ cells in the 4, 5, and 6 mg IDPN/g body weight-treated utricles. Graph (g) shows changes in Notch signaling genes at D1, D5, D6, D8, and D10 after 6 mg IDPN/g body weight injection. Graph (h) shows changes in Wnt signaling genes after IDPN injection. Graph (i) shows changes in Atoh1 and p27kip1 expression after IDPN injection. All hair cell development-related vital genes showed a fluctuating change. The scale bars indicate 20 μm; and .